Vol. 7, Issue 9 (2018)
Qualitative and quantitative phytochemical profile and in vitro anti-oxidant activity of methanolic extract of Artemisia vulgaris
Gandham Sandeep Kumar, Chintha Venkataramaiah and Wudayagiri Rajendra
The present study is aimed to identification and quantification of phytoconstituents and to evaluate the in vitro antioxidant activity of methanolic extract of Artemisia vulgaris. Qualitative and quantitative analysis of phytoconstiuents was performed with methanolic extract of Artemisia vulgaris. Antioxidant activity of extracts was evaluated by estimating H2O2 scavenging activity, DPPH assay, Iron chelating activity and DNA protection assay. Active phytoconstiuents were identified in GC-MS and LC-MS. Physico-chemical properties of phytocostituents were predicted using computational tools. Qualitative analysis revealed the presence of phenols, flavanoids, tannins, steroids, carbohydrates and saponins. GC-MS and LC-MS analysis revealed the presence of 27 compounds. Crude extract contains 360 mg/gm and 110 mg/gm of phenols and flavonoids respectively. Whereas saponin and alkaloid contents were 2.8% and 1.5% W/V respectively. DPPH and H2O2 scavenging activities were 67% and 69% percent respectively. DNA protection assay of Plant extract showed maximum protection at 80µg of crude extract. From the results it is concluded that methanolic extract of Artemisia vulgaris is a good source of potential antioxidant compounds.
How to cite this article:
Gandham Sandeep Kumar, Chintha Venkataramaiah, Wudayagiri Rajendra. Qualitative and quantitative phytochemical profile and in vitro anti-oxidant activity of methanolic extract of <em>Artemisia vulgaris</em>. Pharma Innovation 2018;7(9):348-354.