Step-up in liquid chromatography from HPLC to UPLC: A comparative and comprehensive review
Sahani Kamal and Wakode Sharad
Liquid chromatography (LC) is a powerful tool in separation science employed for clinical research as well as for therapeutic drug monitoring. It is the most widely applied technique to identify, quantify and purify the components from a mixture in which the separation process is based upon the rate of elution of components through a stationary phase typically over a mobile phase gradient using high pressures (600–6000 psi) to push the mobile phase solvents through a stationary phase column. The rate of elution depends on the affinity of the mixture components to the packing material of utilized stationary phase. A purely new system design with advanced technology has been developed, called ultra-high performance liquid chromatography (UPLC), which has been evolved from high performance liquid chromatography (HPLC). The advantages including shorter analysis time, method sensitivity, repeatability and drug specificity justify the wide use of LC techniques in almost every analytical and bio-analytical laboratory. This review focuses on the invention of new LC technique, comparative performance of UPLC and HPLC, and summarizes their application on method development and validation in pharmaceutical analysis over the last few years
How to cite this article:
Sahani Kamal, Wakode Sharad. Step-up in liquid chromatography from HPLC to UPLC: A comparative and comprehensive review. Pharma Innovation 2018;7(8):342-347.