Vol. 7, Issue 7 (2018)

Author(s):
David Kamei, Sobita Devi Simon and Abhilasha A Lal

Abstract:
Indigenous plant of Manipur viz., Flogacanthus Thyrsiflorus, Mariandra benghalensis, Millettia pachycarpus, Allium hookerii and Solanum incanum were test against Helminthosporium oryzae at different concentration i.e., 10%, 15% and 20% in broth and solid media. In-vitro test revealed, maximum significant reduction biomass (dry wt. mycelium) was found at 20% conc. Solanum incanum (0.10 g) with per cent reduction (64.47) followed by Allium hookerii (0.12 g) with biomass reduction (53.57%) over the untreated Control. In solid media maximum significant inhibition on radial growth of mycelium was recorded at 20% conc. S. incanum (3.78 cm) with per cent inhibition 59.81 followed by A. hookerii (4.94 cm) with per cent inhibition 49.62 in compare with others including Control. Probit analysis revealed among selected botanicals Solanum incanum was found most effective with lethal dose (L₅₀) inhibiting 50% biomass production of H.oryzae with lowest probable concentration of 3.08ppm followed by A. Hookerii at 3.1ppm. Likewise in solid media S. incanum was most effective with L₅₀ against the growth of H. oryzae inhibiting 50% at lowest probable concentration of (8.52ppm) followed by A.hookerii (10.65ppm).