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Vol. 7, Issue 7 (2018)

Biochemical studies in experimentally Salmonella Gallinarum infected broiler chicken intoxicated with alpha-cypermethrin

Author(s):
Vikash Sharma, KK Jakhar, Sakshi Tiwari, Vikas Nehra and BL Jangir
Abstract:
Biochemical studies were conducted on two hundred and thirty, day old broiler chicken experimentally infected with Salmonella Gallinarum and exposed to alpha-cypermethrin. These chicks at the age of 7 days were divided randomly into three groups viz. group A, B and C containing 70, 80 and 80 chicks, respectively. All the birds of group B and C were given alpha-cypermethrin @ 63.94 mg/kg bwt (1/5th of MTD) and @ 31.97 mg /kg bwt (1/10th of MTD), respectively orally through drops throughout the experiment. The group A was kept as control i.e. no alpha-cypermethrin was given. At the age of 14 days, the birds of groups B and C were divided into two subgroups (group B into B1 and B2, group C into C1 and C2) of 40 birds each. Similarly, birds of group A were divided into two subgroups A1 and A2 of 30 and 40 birds, respectively. Thereafter, each bird of subgroup A2, B2 and C2 were injected intraperitoneally 107 CFU Salmonella Gallinarum in 1 ml normal saline solution. Blood samples were collected from six birds of each subgroup directly from heart subsequently on 0, 7th, 14th, 21st and 28th day post infection in sterile tubes for serum separation for biochemical parameters analysis. Serum samples were stored in aliquots at -20oC until its use for different biochemical parameters. Biochemical studies revealed significantly higher mean serum AST, ALT, LDH, ALP activities, creatinine concentration and significantly lower total protein and albumin concentration in all the infected groups as compared to control group.
Pages: 325-330  |  717 Views  75 Downloads


The Pharma Innovation Journal
How to cite this article:
Vikash Sharma, KK Jakhar, Sakshi Tiwari, Vikas Nehra, BL Jangir. Biochemical studies in experimentally <em>Salmonella</em> Gallinarum infected broiler chicken intoxicated with alpha-cypermethrin. Pharma Innovation 2018;7(7):325-330.
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