Vol. 7, Issue 7 (2018)

Author(s):
Vikash Sharma, KK Jakhar, Sakshi Tiwari, Vikas Nehra and BL Jangir

Abstract:
Biochemical studies were conducted on two hundred and thirty, day old broiler chicken experimentally infected with Salmonella Gallinarum and exposed to alpha-cypermethrin. These chicks at the age of 7 days were divided randomly into three groups viz. group A, B and C containing 70, 80 and 80 chicks, respectively. All the birds of group B and C were given alpha-cypermethrin @ 63.94 mg/kg bwt (1/5^th of MTD) and @ 31.97 mg /kg bwt (1/10^th of MTD), respectively orally through drops throughout the experiment. The group A was kept as control i.e. no alpha-cypermethrin was given. At the age of 14 days, the birds of groups B and C were divided into two subgroups (group B into B₁ and B₂, group C into C₁ and C₂) of 40 birds each. Similarly, birds of group A were divided into two subgroups A₁ and A₂ of 30 and 40 birds, respectively. Thereafter, each bird of subgroup A₂, B₂ and C₂ were injected intraperitoneally 10⁷ CFU Salmonella Gallinarum in 1 ml normal saline solution. Blood samples were collected from six birds of each subgroup directly from heart subsequently on 0, 7^th, 14^th, 21^st and 28^th day post infection in sterile tubes for serum separation for biochemical parameters analysis. Serum samples were stored in aliquots at -20^oC until its use for different biochemical parameters. Biochemical studies revealed significantly higher mean serum AST, ALT, LDH, ALP activities, creatinine concentration and significantly lower total protein and albumin concentration in all the infected groups as compared to control group.