Cytoscreening of Indigenous pigs for Numerical Chromosomal Abnormalities
Author(s):
Vandana Yadav, NR Sahoo, Pushpendra Kumar, GK Gaur, AP Sahoo and BL Saini
Abstract:
The chromosomal abnormalities are found almost in all the domestic animal species. They reduce not only the production performance but also the reproduction potential of the animal. The present study was designed to investigate the indigenous pigs for the various chromosomal abnormalities. For the present investigation twenty indigenous pigs 10 males and 10 females of same generation and age from the AICRP unit of Pigs, at IVRI Bareilly were selected. Apparently healthy animals and also those with the low litter size (<6) and failure to conceive were used for this study. These animals were screened for numerical chromosomal abnormalities if any, by karyotyping. Preparations of chromosomes for cytoscreening of animals was done on the basis of short-term whole blood lymphocyte culture technique as described by Moorhead and then the karyotypes of animals were prepared using cells arrested at metaphase division. Ten metaphases were used for karyotyping per animal. So, in this study a total of 200 metaphase plates were used for screening 20 animals. All the animals were found to be devoid of any numerical chromosomal abnormality (aneuploidy and euploidy) as per cytoscreening.
How to cite this article:
Vandana Yadav, NR Sahoo, Pushpendra Kumar, GK Gaur, AP Sahoo, BL Saini. Cytoscreening of Indigenous pigs for Numerical Chromosomal Abnormalities. Pharma Innovation 2018;7(7):14-17.