Vol. 7, Issue 6 (2018)

Author(s):
Shivkumar, Ajeet Kumar, AK Singh, M Honparkhe, Sumit Singhal, Gurpreet Singh and Prahlad Singh

Abstract:
The present study was conducted to minimize the oxidative stress in subfertile buffalo bulls by oral supplementation of herbal mixture. The study was conducted in three phases of 60 days each. Three subfertile buffalo bulls were supplemented with herbal mixture containing Panax ginseng roots, Shilajit, Withania somnifera roots, Tribulus terrestris fruits, Turnera diffusa leaves; Ptychopetalum olacoides bark each @ 400 mg/100 kg body weight and Pausinystalia yohimbe bark @ 300 mg/100 kg body weight of bulls. Blood samples were collected once in a week during all the three phases. Lipid peroxidation (MDA), superoxide dismutase (SOD) activity and glutathione peroxidase (GPx) were estimated in blood samples. Herbal supplementation significantly (P<0.05) reduced the lipid peroxidation in supplementation (401.18 ± 20.06 μmole/gm Hb) and post-supplementation (259.69 ± 11.35 μmole/gm Hb) phases. The activity of SOD increased during supplementation (982.50 ± 111.63 U/gm Hb/min) and post-supplementation (1632.71 ± 140.57 U/gm Hb/min) phases. GPx activity was significantly (P<0.05) increased only during supplementation phase (35.17±6.06 U/gm Hb/min). In conclusion, the supplementation of herbs reduces the oxidative stress in subfertile buffalo bulls.