Vol. 7, Issue 6 (2018)
Effect of solvent extraction system on the antioxidant activity and RP-HPLC based determination of phenolic and water soluble vitamins in an annual herb Mazus pumilus
Sudeshna Datta, Tapan Seal, BK Sinha and Soumen Bhattacharjee
The objective of the present study was to evaluate the content of water-soluble vitamins like ascorbic acid (C), thiamine (B1), riboflavin (B2), niacin (B3), pantothenic acid (B5), pyridoxine (B6) and folic acid (B9) in an ethno-medicinally edible plant Mazus pumilus Burm.f. by high performance liquid chromatography (HPLC) method. The in vitro antioxidant activity was carried out in different solvent system of varying polarity. The 70% Aqueous (Aq.) ethanol was appeared to be the most potent solvent for extraction of antioxidant components. The total phenolic and flavonoid content were found 65.640 ± 0.130mg/g dm (dry mass) and 18.877 ± 0.070mg/g dm respectively in the plant. The estimation of phenolic acids, polyphenolics in the 70 % aq. ethanol extract was also carried out by HPLC method. The water soluble B vitamins ranged between 0.072 to 5.632 mg/100g dm. The results confirm that this ethno medicinally important plant is a good source of natural antioxidants which could be used for the good of human being at the same time it can be used as a vitamin supplement to our diet. The present study also gives an account of traditional significance of the plants under investigation.
How to cite this article:
Sudeshna Datta, Tapan Seal, BK Sinha, Soumen Bhattacharjee. Effect of solvent extraction system on the antioxidant activity and RP-HPLC based determination of phenolic and water soluble vitamins in an annual herb Mazus pumilus. Pharma Innovation 2018;7(6):09-15.