Vol. 7, Issue 5 (2018)
Improvement in post-thaw semen quality by minimizing the lipid peroxidation following herbal treatment in sub fertile buffalo bulls
Shiv Kumar, Ajeet Kumar, AK Singh, Mrigank Honparkhe, Prahlad Singh and Puneet Malhotra
The present experiment was conducted to study the impact of herbal treatment on post thaw semen quality and lipid peroxidation level in subfertile buffalo bulls. Three Murrah subfertile buffalo bulls maintained at bull station, Guru Angad Dev Veterinary and Animal Sciences University (GADVASU), Ludhiana, India (Latitude/Longitude, 30.55°N, 75.54° E) with the history of poor semen quality (pre-freeze individual motility < 60%, post-thaw individual motility < 40%, Viability : < 70% and Abnormality: > 20%) were orally supplemented with herbal mixture (Panax ginseng roots, Shilajit, Withania somnifera roots, Tribulus terrestris fruits, Turnera diffusa leaves, Ptychopetalum olacoides bark of each 400 mg/100 kg body weight and 300 mg/100 kg body weight of Pausinystalia yohimbe bark) daily for 60 days of treatment phase. Two semen ejaculates per week per bull were collected during pre-treatment, treatment and post-treatment phases of 60 days each and extended with Tris egg yolk extender. The freezing of extended semen was carried out in a biofreezer (i.e. 4 °C to -15 °C @-30 °C/min, -15 °C to -140 °C @-50 °C/min). Further, the post thaw semen was assessed in terms of individual motility, viability, total sperm abnormalities, mitochondrial membrane activity (TMRM fluorescent dye), intact acrosome and plasma membrane integrity. Further, the lipid peroxidation level was assessed in terms of malondialdehyde (MDA). During the treatment and post-treatment phase, individual motility, viability, total sperm abnormalities, mitochondrial membrane activity and intact acrosome and plasma membrane integrity were significantly (P<0.05) higher. Moreover, lipid peroxidation was significantly (P<0.05) lower during treatment phase and it remained similar (P>0.05) during the post-treatment phase in contrast to pre-treatment phase.
How to cite this article:
Shiv Kumar, Ajeet Kumar, AK Singh, Mrigank Honparkhe, Prahlad Singh, Puneet Malhotra. Improvement in post-thaw semen quality by minimizing the lipid peroxidation following herbal treatment in sub fertile buffalo bulls. Pharma Innovation 2018;7(5):240-244.