Epididymal semen analysis in testicular toxicity of doxorubicin in male albino wistar rats and its amelioration with quercetin
S Divya, D Madhuri, M Lakshman and A Gopal Reddy
A total of 48 male albino Wistar rats were randomly divided into 4 groups consisting of 12 in each group. Group 1-Control, Group 2-Doxorubicin treated @ 4mg/kg b.wt intraperitoneally weekly once. Group 3-treated with quercetin @ 80mg/kg b.wt, orally daily. Group 4-treated with doxorubicin @ 4 mg/kg b.wt, intraperitoneally weekly once and quercetin @ 80mg/kg b.wt, orally daily. Six rats from each group were sacrificed by cervical dislocation at fortnight intervals of experimental study and the abdominal cavity was opened through a midline abdominal incision to expose the reproductive organs. The cauda epididymis of one testis was excised to obtain the epididymal contents and semen analysis was done. There was significant increase in abnormal sperm count and significant decrease in sperm motility and sperm count in Group 2 animals as compared to other groups. Further sperm count was increased in Group 3 as compared to control Group 1 showing quercetin improves fertility in male animals.
How to cite this article:
S Divya, D Madhuri, M Lakshman, A Gopal Reddy. Epididymal semen analysis in testicular toxicity of doxorubicin in male albino wistar rats and its amelioration with quercetin. Pharma Innovation 2018;7(4):555-558.