Vol. 7, Issue 4 (2018)

Author(s):
Shankaraiah P, Swathi B, Aruna Kumari G, Priyanka B, Srinivasa Prasad CH, Raju J and Parthasarathi T

Abstract:
An experiment was conducted to establish in vitro maturation protocol for production of MII stage oocytes from preantral follicles. Goat preantral follicles (PFs) (diameter ranging from 150-400µ) were cultured in culture medium to support growth and development in vitro for a period of eight days. After culture period of 8 days, oocytes were retrieved from cultured preantral follicles (T₁-T₅), were kept for in vitro maturation for a period of 27hrs in different IVM media (IVM-I, IVM-II, IVM-III and IVM-IV) and antral oocytes were kept for IVM as control-II in different IVM media. After 27 hours of in vitro maturation period, oocytes were denuded and evaluated for nuclear status by staining with propidium iodide. Among in vitro maturation protocols of our study the proportion of oocytes exhibiting M-II stage were maximum in IVM-III. And among treatments T₃ (T₄+FSH+GH+EGF) had a highest percentage of M-II stage in all IVM media and these results were statistically different (P≤0.05) with oocytes of other treatments. From the results of the present study, it can be concluded that, in vitro development of goat PFs could be significantly improved through the addition of complement inactivated caprine follicular fluid to the in vitro maturation media.