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Vol. 7, Issue 4 (2018)

Effect of different concentrations of kisspeptin on in vitro maturation rate of buffalo oocytes

Author(s):
Kolipaka Rajesh, B Swathi, G Aruna Kumari and M Shanmugam
Abstract:
Culture grade oocytes collected from slaughtered healthy buffaloes were randomly allotted to 4 treatment (T) groups containing oocyte maturation media and were allowed to mature at 38.5oC and 5% CO2 in humidified atmosphere for 22 hours. Tissue Culture Medium 199 (TCM 199B), supplemented with Gentamicin was used as control (T1), while for groups T2, T3 and T4 kisspepetin (Kp) was supplemented to control media at 5 µg/ml, 10 µg/ml and 15 µg/ml respectively. The oocytes were evaluated based on the percentages of Cumulus Cell Expansion (CCE) and 1st Polar Body extrusion (PB). Addition of Kp to the maturation media observed that the proportion of oocytes showing CCE and PB was significantly (P ≤ 0.5) higher when compared to the control. Among all the three concentrations of Kp, the proportion of oocytes showing the percentage of CCE and PB was highest in T3 (73.21% and 22.11%, respectively) compared to the T2 and T4. Inferring that 10 µg/ml concentration of Kp would yield optimum in vitro maturation of buffalo oocytes.
Pages: 375-378  |  721 Views  158 Downloads


The Pharma Innovation Journal
How to cite this article:
Kolipaka Rajesh, B Swathi, G Aruna Kumari, M Shanmugam. Effect of different concentrations of kisspeptin on in vitro maturation rate of buffalo oocytes. Pharma Innovation 2018;7(4):375-378.

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