Toll Free Helpline (India): 1800 1234 070

Rest of World: +91-9810852116

Free Publication Certificate

Vol. 7, Issue 4 (2018)

A direct PCR approach for gene amplification from whole blood sample of sheep

Author(s):
Kontham Sampath, Vishweshwar Kumar Ganji and Pottabathula Mallesh
Abstract:
The genomic DNA extraction and PCR amplification of gene fragment from whole blood sample is a cumbersome process. Here in this study we described a direct PCR amplification of gene fragment from the whole blood sample of sheep bypassing the genomic DNA extraction steps. The detailed technique is explained and the results are suggestive of high accuracy in gene amplification. This can be used to cut short the time and is economic for gene amplification from whole blood sample with the same available reagents as for the conventional method.
Pages: 167-168  |  1068 Views  114 Downloads


The Pharma Innovation Journal
How to cite this article:
Kontham Sampath, Vishweshwar Kumar Ganji, Pottabathula Mallesh. A direct PCR approach for gene amplification from whole blood sample of sheep. Pharma Innovation 2018;7(4):167-168.

Call for book chapter