Vol. 7, Issue 4 (2018)

Author(s):
Meenakshi Basera, Amit Chandra, Vandana A Kumar and Atul Kumar

Abstract:
Potato is an important tuber crop grown extensively for consumption as staple food or for industrial uses. Seed generally accounts as the costliest input in potato cultivation that is often marred by poor quality or infections. In vitro multiplication of potato constitutes one of the most lucrative alternate options to circumvent the tuber borne problems in vegetative propagation. Hormones have profound effect on growth and differentiation of in vitro cultures. In this study we have showed the effect of brassinosteroids on in vitro culture and the subsequent growth of potato. We found that number of nodes, number of branches, shoot length, number of leaves, culture survival were highest under cultures supplied with Gibberellic acid (GA) + Napthaleneacetic acid (NAA) + Epibrassinolide (EBL) at 0.5 µM +0.1 µM +0.1 µM concentration mixture. Shoot initiation was also earlier when cultures were supplied with GA+NAA+EBL (0.5 µM + 0.1 µM + 0.1 µM). We conclude that by the study that the effect of EBL in comparison to GA+NAA, length of shoots, numbers of nodes and leaves were significantly higher when GA+NAA were supplemented with EBL.