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Vol. 7, Issue 3 (2018)

A low cost, high throughput gel electrophoresis method for separation of SSR markers in Aloe vera

Author(s):
Ravinder Kumar, Raj Kumar Salar, Ashok Kumar, Anil Kumar and Vinod Chhokar
Abstract:
Aloe vera is the important commercial crop used for medicinal purposes from ancient time. Because of the limitless medicinal value, it is necessary to study the genetic diversity in Aloe vera to detect the commonness and distinctness of the genotypes for the improvement of the traits. The main objective of the present investigation is to compare the gel-based electrophoresis methods for the separation of simple sequence repeats markers in Aloe vera. Proper separation of simple sequence repeats alleles is very critical that differ a little as two to five base pair. In the present study, eighteen genotypes of Aloe vera were screened using five di-nucleotide simple sequence repeats markers. Four methods for the separation of microsatellite markers were used i.e. simple agarose gel, metaphor agarose gel, polyacrylamide gel on midi assembly, polyacrylamide gel on sequencing gel apparatus. Among these, the best separation of alleles was observed in polyacrylamide gel on sequencing gel apparatus and least separation was observed in case of simple agarose gel electrophoresis. A total number of alleles detected for five microsatellite markers were 114, 151, 162 and 165 in standard agarose gel, metaphor agarose gel, polyacrylamide gel on midi assembly, polyacrylamide gel on sequencing gel apparatus respectively. Therefore, polyacrylamide gel on sequencing gel apparatus can be used as an efficient technique for the best resolution of the microsatellite markers.
Pages: 622-627  |  355 Views  10 Downloads
How to cite this article:
Ravinder Kumar, Raj Kumar Salar, Ashok Kumar, Anil Kumar, Vinod Chhokar. A low cost, high throughput gel electrophoresis method for separation of SSR markers in Aloe vera. Pharma Innovation 2018;7(3):622-627.
The Pharma Innovation Journal