Vol. 7, Issue 3 (2018)

Author(s):
Vishweshwar Kumar Ganji, Sampath Kontham, Mallesh Pottabathula and Dechamma HJ

Abstract:
In molecular biology, we most often handle with bacmid when working with baculovirus expression system. The cloning involves a two-step process and the recombinant bacmids are screened by blue-white lac operon system, white colonies are thought to be positive for transposition. But in our study with PCR based approach, we found that not all the white colonies are recombinants. Hence, it is necessary to be cautious while screening for recombinant bacmids and may be it is good too screen by two-way approach, by blue-white lac operon system followed by PCR approach using one bacmid specific and one gene specific primers.