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Vol. 7, Issue 2 (2018)

Identification of primary ossification centers in the forelimbs of buffalo fetus by modified alizarin red – s method

Author(s):
E Raja Ravi Teja, N Rajendranath and G Purushotham
Abstract:
In this modified alizarin red-s method, the fetuses were fixed in 10 percent Neutral buffered formalin for 1 week. Potassium hydroxide digestion time was reduced to secure the muscular system and then stained with alizarin red-s. Excess stain was removed by fresh 4 percent potassium hydroxide solution. Stained fetuses were cleared for 2 weeks by using two clearing agents. The primary ossification centers of the forelimbs were clearly visible. The fetuses were preserved in glycerin.
Pages: 246-247  |  567 Views  5 Downloads
How to cite this article:
E Raja Ravi Teja, N Rajendranath, G Purushotham. Identification of primary ossification centers in the forelimbs of buffalo fetus by modified alizarin red – s method. Pharma Innovation 2018;7(2):246-247.
The Pharma Innovation Journal