Vol. 7, Issue 2 (2018)

Author(s):
E Raja Ravi Teja, N Rajendranath and G Purushotham

Abstract:
In this modified alizarin red-s method, the fetuses were fixed in 10 percent Neutral buffered formalin for 1 week. Potassium hydroxide digestion time was reduced to secure the muscular system and then stained with alizarin red-s. Excess stain was removed by fresh 4 percent potassium hydroxide solution. Stained fetuses were cleared for 2 weeks by using two clearing agents. The primary ossification centers of the forelimbs were clearly visible. The fetuses were preserved in glycerin.