Vol. 7, Issue 12 (2018)

Author(s):
Subhendu Sekhar Mishra and Ashish Kumar Sharma

Abstract:
The mucoadhesive gel formulations are helpful to prolong the residence time at the nasal absorption site and thereby facilitate the uptake of drug. The objective of the present study was to develop a thermo sensitive in Situ nanogel system based on chitosan and tripolyphosphate for nasal delivery of insulin. Nanogel containing insulin was prepared through an ionic gelation method. The concentration of the components was optimized during formulation development and then characterized in terms of Drug content, mucoadhesive strength, pH, Spreadability, and stability study and drug release behavior. The drug release results were fitted on five mathematical models to choose the model best describing the phenomenon. The in vitro release of insulin from gel network was observed spectrophotometrically which was good enough to maintain blood glucose level for 14 hour. Then the nano-formulation and insulin Sc injection as control were administered in the nasal cavity for rats and after 2, 4, 6, 8 and 10 hrs their blood glucose levels, serum insulin level analyzed for antidiabetic activity. The observed in vitro and in vivo results indicate that the proposed thermo sensitive in Situ gelling system has substantial potential as nasal delivery system for insulin.