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Vol. 7, Issue 12 (2018)

Antioxidant and GC-MS analysis of Thamnolia subuliformis (Ehrh.) W.L. Culb. from western Himalaya

Author(s):
Vartika Pant and PB Rao
Abstract:
Lichens are the unique organism in the plant kingdom and produces different types of secondary metabolites than in higher plants. These different metabolites along with other chemical compounds can be utilized for curing aches and diseases. In the present study, extraction yield (Soxhlet method), qualitative screening of biochemicals (alkaloids, carbohydrates, fats, flavonoids, phenolics, proteins, saponins, steroids and tannins), antioxidant activity using different assays (DPPH, TAA, FCA and Reducing power activity), TPC and TFC in different solvents (acetone, ethanol and methanol) along with GC-MS analysis was performed on a macrolichen, T. subuliformis. Among the solvents, the methanol showed comparatively higher activity than in other two solvents. The yield obtained was higher than previous studies. The biochemical screening reveals the presence of all selected biochemicals except alkaloids and steroids. Among all the experiments performed methanol showed comparatively better results than acetone and ethanol. The GC-MS analysis of the extract showed the presence of the 60 different biochemicals out of which, hexadecanoic acid, methyl ester, phytol and evernic acid, etc., are reported to possess anticancer, anti-inflammatory, antimicrobial, antioxidants, diuretic and hypocholesterolemic activities. Thus, T. subuliformis is a potent source of antioxidants and nutraceutical as well as functional food applications.
Pages: 82-88  |  698 Views  28 Downloads
How to cite this article:
Vartika Pant, PB Rao. Antioxidant and GC-MS analysis of <em>Thamnolia subuliformis</em> (Ehrh.) W.L. Culb. from western Himalaya. Pharma Innovation 2018;7(12):82-88.
The Pharma Innovation Journal