Vol. 7, Issue 11 (2018)

Author(s):
Shital S Phuse and Zia H Khan

Abstract:
The present work was aimed to determine thin layer chromatographic screening and antioxidant properties of leaves and flower extracts of Caesalpinia pulcherrima. Ethyl acetate and acetone extracts were screened for identification of phenolic and flavonoids. Thin layer chromatography (TLC) was performed first to confirm the qualitative characterization of phytochemical screening. Antioxidant activity of the extracts was tested qualitatively with Superoxide radical scavenging assay. Phytochemical screening test showed that both ethyl acetate and acetone fractions from the plant materials contain phenolic and flavonoids and exhibited higher antioxidant activity in comparison of Standard Ascorbic acid. Thin Layer chromatography of the ethyl acetate and acetone were showed colour spots with different RF values under UV light which confirmed the presence of phytochemicals and antioxidants. IC50 values of the plant material extracts revealed the potential of antioxidant activity of this plant. Thus these findings concluded that, this can be further investigated for isolation and identification of active secondary metabolites of medicinal utilities.