Isolation, morphological and biochemical characterization of antibiotic producing bacteria from various sewage sites of Visakhapatnam, Andhra Pradesh
VVSR Krishna Kishore and M Vijayalakshmi
The emergence of antibiotic resistance among pathogens and need for better, broad spectrum antibiotics is always in high demand. Hence isolation of antibiotic producing bacterial strains from sewage was carried out in the laboratory. In the present study, antibiotic producing bacteria were isolated from various sewage sample collected from Visakhapatnam. After primary screening, three bacterial strains (AntC1, AntC3 and AntC8) were isolated, which showed antimicrobial activity against some common bacteria namely, E. coli, and S. aureus. The isolated strains showed prominent zones of inhibition against E. coli and S. aureus. To identify the isolated strains, Microscopic examination and biochemical tests were performed and it was found that the strains were related to be Bacillus species with some differences in cultural characteristics. Identification of those strains by 16s rRNA gene sequencing will be useful in improving these strains for antibiotic production.
How to cite this article:
VVSR Krishna Kishore, M Vijayalakshmi. Isolation, morphological and biochemical characterization of antibiotic producing bacteria from various sewage sites of Visakhapatnam, Andhra Pradesh. Pharma Innovation 2018;7(11):287-291.