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Vol. 7, Issue 10 (2018)

Rp-HPLC technique for the assay of olmesartan and Rosuvastatin simultaneously: development and validation of method

Author(s):
Burla Sunitha Venkata Seshamamba
Abstract:
An isocratic elution mode RP-HPLC method is described for the determination of olmesartan (OST) and Rosuvastatin (RST) simultaneously in pure drug and pharmaceutical formualtions. Chromatography was achieved on BDS C18 column, with a mobile phase consisting of 0.01M sodium dihydrogen orthophosphate (60%) and methanol (40%). Analysis of the analytes was carried out at 265 nm using photodiode array detector. Under optimized chromatographic conditions, the retention times were 3.138 min and 3.827 min for OST and RST, respectively. The peak areas measured was related to OST and RST concentration. Linearity was obtained in concentration ranges of 10-30 μg/ml (RST) and 20-60 μg/ml (OST). The limits of detection (LOD) and quantification (LOQ) were 0.05 and 0.14 μg/ml for OST and 0.002 and 0.005 μg/ml for RST. The relative standard deviations for OST and RST were <1.0%, and the respective percentage recoveries were in the range of 99.31-99.82% (OST) and 100.17-101.48% (RST), implying good precision and accuracy of the method. The proposed method was effectively applied to the estimation of OST and RST in tablet samples with good accuracy and precision. Therefore, the method could be considered as a suitable tool for combined analysis of OST and RST.
Pages: 669-675  |  516 Views  99 Downloads


The Pharma Innovation Journal
How to cite this article:
Burla Sunitha Venkata Seshamamba. Rp-HPLC technique for the assay of olmesartan and Rosuvastatin simultaneously: development and validation of method. Pharma Innovation 2018;7(10):669-675.

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