Protoplasts isolation of soy bean Glycine max (L.) Merrill from leaf explants
Parvathi D, Venkateshwarlu M, Dinesh Khanna N and Ugandhar T
A simple protoplast isolation protocol that was designed to recover totipotent plant protoplasts with relative ease has been described· The key elements of the protocol are, tissue digestion at slightly elevated temperatures and use of protoplast releasing enzymes that are stable and efficient at higher temperatures· Besides enzymes, the protoplast isolation cocktail consisted of an Osmoticum (Mannitol or MgSO4), and a Protectant (CaCl2 2H2O), all dissolved in distilled water. The protocol has ensured reproducibility, higher yields and is gentle on protoplasts as the protoplasts obtained were amenable to cell wall regeneration and cell division· Plant regeneration was demonstrated for Glycine maxo L. from protoplasts isolated by this method· Wall regeneration and cell division were obtained in other species. The merits of the protocol are, simple and easy-to-handle procedure, non-requirement of preconditioning of donor plant and explants, incubation without agitation, satisfactory yields, cultivability of the protoplasts isolated and applicability of the protocol to a large number of species including mucilage-containing plants.
How to cite this article:
Parvathi D, Venkateshwarlu M, Dinesh Khanna N, Ugandhar T. Protoplasts isolation of soy bean Glycine max (L.) Merrill from leaf explants. Pharma Innovation 2018;7(10):661-665.