Synthesis of nanosilver (NS) using microbes has acquired insightful interest as they deserve potential to synthesize noble metal nanoparticles of various shape and size. In the present study, NS was synthesized by a bacterial strain isolated from heavily metal contaminated soil.
Phenotypic identification showed that isolated was strain may be from the category of Bacillus sp. Treatment of this isolated bacteria with 1 mM AgNO3, showed to have its ability to form NS extracellularly at elevated temperature within 5 minutes. Synthesis of visual observation inferred synthesis of NS, further confirmation by UV–Visible absorption was done which gave surface plasmon characteristic peak at 450 nm. Subsequently, characterization of NS was done by transmission electron microscopy which revealed the size of NS was in the range of 50-100 nm. Present study was a preliminary study to reflect the possibility of isolation of bacteria from heavily metal contaminated soil and its subsequent application in the synthesis of NS which revealed the fulfillment of the objective.