Vol. 7, Issue 10 (2018)

Author(s):
Pukhraj Meena

Abstract:
Caffeine (1, 3, 7-trimethylxanthine) increases calcium excretion in the urine and so heavy caffeine usage may increase the risk of osteoporosis. Biosensor adoption is increasing every year and the number of biosensor applications is continuously growing. In addition reproducibility of the biosensor is very good and the biosensor can be used as an alternative method for routine analysis of caffeine. The interference effects of the other compounds were negligible. Developed biosensor gold screen printed electrode (GSPE) was also used in real sample analysis. Our study showed minimum response detection ranges with lowest detection limit (LOD) than previous literatures for the same purpose of study and response time was very short just only 40 s by using of isolated screen printed gold electrode for the development of amperometric biosensor against caffeine over concentration range. Caffeine in the soft drinks can be determined sensitively using the biosensor. In this study, it was found that present amperometric biosensor having of 95-100% recovery which was average result by comparing with another methods such as Voltammetry, Flow injection, SFC-FTIR, MIP–PMAA/PVC sensor & UV-VIS (AOAC 12.028).