Vol. 7, Issue 10 (2018)

Author(s):
Amrutha VS, Vinodkumar K, Sulficar S, Vijayakumar K and Priya PM

Abstract:
Present study was done for the detection of enetropathogenic E. coli (EPEC) induced diarrhoea in neonatal calves and analysis of haemato-biochemical changes during the disease. Genes for production of the virulence factor Intimin was detected by PCR in DNA extracts of faecal samples from six out of 50 diarrhoeic calves. Haematology of affected calves revealed anaemia (VPRC 20.27 per cent) and pan leukocytosis (mean TLC 13533cells/mm³). Biochemical analysis of serum showed hyperlacticaemia (2.606 mmol/L) and hyponatraemia (137.167 mmol/L). Hypoalbuminaemia (1.415 g/dl) and decrease in level of A: G ratios (0.539) were the two other prominent variations. No other significant alterations could be detected in any other clinical or haemato-biochemical parameters.