Quantitative determination of phytochemical constituents and evaluation of acute antihyperglycemic activity of four various extracts of Cleome viscosa whole plant in STZ-induced diabetic rats
Yarrappagaari Suresh, Gutha Rajasekar, Benne Lakshmi Narsimhulu, Thopireddy Lavanya, Gundala Harold Philip and Saddala Rajeswara Reddy
Cleome viscosa is traditionally used in the management of diabetes mellitus. While this claim has not been investigated technically, the aim of this study was to estimate the phytochemical constituents and investigate the antidiabetic property of the methanolic extract (MeCV), aqueous extract (AqCV), ethyl acetate (EaCV) and n-hexane (NhCV) extract of Cleome viscosa whole plant in streptozotocin (STZ)-induced diabetic rats. Diabetes was induced by using a single intra peritoneal injection of STZ (45 mg/kg b.w.). Determination of FBG levels at 0 hour (before administration of extract) and 1 h, 3 h, 5 h and 7 h (after administration of extract) by using accucheck glucometer. Quantitative determination of phytochemicals was done by using standard methods. We observed, MeCV showed a maximum effectiveness with a dosage of 400 mg/ kg b.w. at 5 h which is about -300.38±42.91% diminution in FBG level over zero time values. The FBG-decreasing effect of MeCV extract was more rapid than that of the remaining three extracts when compared to diabetic control; the diminishing rates were -50.70±2.22% after 1h, -179.42±33.31% after 3 h, -300.38±42.91% after 5 h and -92.64±16.08% after 7 h. After 5 h (-300.38±42.91%), the effect of MeCV on FBG levels was significantly similar mode to that of reference drug glibenclamide (-324.24±33.21%) in diabetic rats. These results suggest that whole plant of MeCV extract is valuable in the management of diabetes by diminution of blood glucose levels presumably due to the presence of phytochemical constituents such as alkaloids, saponins and tannins.
How to cite this article:
Yarrappagaari Suresh, Gutha Rajasekar, Benne Lakshmi Narsimhulu, Thopireddy Lavanya, Gundala Harold Philip, Saddala Rajeswara Reddy. Quantitative determination of phytochemical constituents and evaluation of acute antihyperglycemic activity of four various extracts of <em>Cleome</em> <em>viscosa</em> whole plant in STZ-induced diabetic rats. Pharma Innovation 2018;7(10):30-35.