Petal callus mediated de novo regeneration of shoots in carnation (Dianthus caryophyllus L.)
Swarupa Jorapur, Neelkantha Jogdande and Dinkar Dhumale
Hormonal combinations and concentrations were examined for optimization of callus induction and regeneration efficiency, using petal explants of carnation varieties Master and Domingo. Callus induction and regeneration response varied with explants. In ‘Master’, enhanced callusing was obtained with Murashige and Skoog, 1962 medium (MS) + 2,4 Dichlorophenoxyacetic acid (2,4-D) 1.5 mg l-1 + α-Naphthaleneacetic acid (NAA) 0.5 mg l-1 whereas in ‘Domingo’, MS + 2,4-D 1.0 mg l-1 + NAA 0.5 mg l-1 was found superior. Petal callus in ‘Master’ regenerated best with MS + 6-benzyl amino purine (BAP) 2.0 mg l-1 + NAA 0.1 mg l-1 only when pH was reduced to 5.6. In ‘Domingo’, enhanced regeneration was obtained with MS + BAP 2.0 mg l-1 + NAA 0.2 mg l-1. Callus regenerants induced roots with half strength MS medium or ½ MS + Indole-3-butyric acid (IBA) 0.5 mg l-1 for ‘Master’ and ½ MS + IBA 1.5 mg l-1 for ‘Domingo’ with 2% sucrose.
How to cite this article:
Swarupa Jorapur, Neelkantha Jogdande, Dinkar Dhumale. Petal callus mediated de novo regeneration of shoots in carnation (Dianthus caryophyllus L.). Pharma Innovation 2018;7(1):218-222.