Vol. 7, Issue 1 (2018)

Author(s):
Sneha Ogale, Karan Singh Yadav and Shrutika Navale

Abstract:
In our study, a total of 13 different bacterial endophytes (KV-1 to KV-13) isolated from Gloriosa superba during a previous study, were tested for different plant growth promoting properties like IAA (Indole-3-acetic acid) production by the method of Gordon and Weber; phosphate solubilisation on Pikovskaya’s medium; siderophore production on Chrome azurol S (CAS) medium; Nitrogen fixation on Jensen’s Nitrogen-free medium; ammonia production using Nessler’s reagent; and Hydrocyanic acid (HCN) production on Nutrient Agar supplemented with 4.4 g/L glycine, having Whatmann No.1 filter paper strip soaked in 2% sodium carbonate in 0.5% picric acid held above it. Two promising isolates (KV-5 and KV-11) among the 13, were positive for all plant growth promoting traits tested except HCN production, whereas KV-13 was positive for phosphate solubilization, siderophore production and nitrogen fixation indicating their importance as potential candidates for the development of bioinoculants.