Toll Free Helpline (India): 1800 1234 070

Rest of World: +91-9810852116

NAAS RATING: 5.23, Impact Factor: RJIF 5.98 | Free Publication Certificate
updates
NAAS Rating: 5.23 new
Vol. 6, Issue 9 (2017)

Development and validation of stability indicating RP-HPLC method for estimation of teriflunomide in active pharmaceutical ingredient

Author(s):
Bhavya Mehta, Pravin Prajapat and Yatin Gohil
Abstract:
Developed method is a rapid, simple and Reverse Phase-High Performance Liquid Chromatography (RP-HPLC) method for determination of teriflunomide. The HPLC separation was achieved on Agilent-1260 Infinity series instrument with Eclipse XBD C18 (Agilent) column (150X4.6 mm, 5µm) using a mobile phase of acetonitrile and di-potassium hydrogen anhydrous (K2HPO4) solution (40:60; v/v) containing TEA (pH 7) at a flow rate of 1.0 ml/min. The method was validated for specificity, linearity, precision, accuracy, robustness and ruggedness. The elution of peak was rapid which s takes about 3.5 minutes. The method was found to be simple, specific, precise, accurate, linear and reproducible. The method can be applied for the quality control of commercial teriflunomide API (active pharmaceutical ingredient) to quantify the drug and to check the formulation content uniformity and purity of drug.
Pages: 440-449  |  972 Views  108 Downloads
How to cite this article:
Bhavya Mehta, Pravin Prajapat, Yatin Gohil. Development and validation of stability indicating RP-HPLC method for estimation of teriflunomide in active pharmaceutical ingredient. Pharma Innovation 2017;6(9):440-449.
The Pharma Innovation Journal