Vol. 6, Issue 9 (2017)

Author(s):
Rakesh Patel, Gopal Rathor, Bhupendra H Bhargav, K Singh and Davendra Bharti

Abstract:
Rhizobium species are group of bacteria that fix atmospheric nitrogen symbiotically and stimulate the growth of plants. In the present study, root nodulating bacteria were isolated from root nodules of Cicer arietinum on yeast extract mannitol agar (YEMA) medium. In conical flask assays of Rhizobium, seed inoculation was more effective than soil inoculation. The effect of inoculation of Rhizobium on nodulation and growth of Cicer arietinum was also investigated using a water agar medium under laboratory conditions. In case of seed germination on the growth of Bengal gram (Cicer arietinum), crop plants, it was noted that no enhanced growth rate was obtained; hence Rhizobium may be used as cobioinoculant with other banificial bacteria and micronutrient. Use of micro and macro nutrient may improve effectiveness of Rhizobium biofertilizers for common bean production. Testing of Rhizobium under field conditions will further elucidate their effectiveness on grain yields of Cicer arietinum in Nimar area with different physical condition and nutrient.