Vol. 6, Issue 9 (2017)

Objectives: To establish HPTLC Fingerprint analysis of chloroform extract of Amaranthus tricolor and Amaranthus viridis herbs.

Materials and Methods: The chloroform extracts of A. viridis (AVC) and A. Tricolor (ATC) were selected for HPTLC analysis. The best resolutions were obtained with solvent system of toluene: ethyl acetate in ratio of 7:3. CAMAG Linomat 5 applicator was used for sample application in volume of 5 and 10 micro litres for both plant extracts AVC and ATC in the form of band on precoated plates. The plates were developed in CAMAG glass Twin Trough Chamber 20x10cm in linear ascending direction. The total numbers of tracks were four. The detection of spots were carried out by using CAMAG TLC Scanner-3 with mercury remission florescence lamps at wavelength of 366 nm. The retention factor (Rf) values and finger print data were recorded by WIN CATS planer chromatography manager software.

Results: The result revealed presence of thirteen components in A. viridis extract (AVC) and fourteen in A. Tricolor extract (ATC).

Conclusion: The main finding of present study was that HPTLC finger printing of A. viridis and A. Tricolor chloroform extracts were first time established. In future these finding could be helpful for herbal drug standardization.