Vol. 6, Issue 9 (2017)

Author(s):
N Mohammad Sharif, B Sreedevi, RK Chaitanya and Ch Srilatha

Abstract:
The study aimed at the detection of extended spectrum beta-lactam (ESBL) resistance in Pseudomonas species isolated from healthy and diarrhoeic dogs in Andhra Pradesh (India). Rectal swabs from healthy (92) and diarrhoeic (44) dogs were subjected for isolation of Pseudomonas species by conventional culture methods and screened for ESBL resistance by disc diffusion method. A total of 46 Pseudomonas isolates were recovered (26/92 of healthy and 20/44 of diarrhoeic dogs). Phenotypic screening revealed resistance to cefotaxime (52.1%), ceftriaxone (23.9%), ceftazidime (28.2%) and aztreonam (13.0%) with an overall incidence of 58.6% (27/46) beta-lactam resistance. Overall incidence of ESBL resistant phenotype was found to be 15.2% (7/46) by combination disc method. Detection of beta-lactamase genes was carried out using a set of three multiplex PCR assays and a single uniplex PCR assay. Predominant beta-lactamase genes detected include bla_OXA (69.5%) followed by bla_TEM (36.9%), blaSHV (26.0%), bla_CTX-M group-1 (15.2%) and blaAmpC (4.34%). All the isolates were found to be negative for bla_ACC, bla_MOX and bla_CTX-M group-2 genes. The present study reporting the first detection of ESBL resistant phenotype in Pseudomonas species of canine origin in India adds to the alarming ESBL resistance reports in the gut microbiota of dogs world-wide.