Vol. 6, Issue 8 (2017)

Author(s):
NP Darzuli, LV Vronska, TA Groshovyi and NM Beley

Abstract:
The study object was medicinal herb - Pyrola rotundifolia leaf. To identify biologically active substances in medicinal plants, they used the thin-layer chromatography (TLC). TLC studies used a chromatographic plate Silica Gel 60 F254 (Mersk, Germany), a chromatographic chamber CAMAG, a samples applicator Linomat 5 (CAMAG, Switzerland), a UV lamp to view chromatograms CAMAG, and standard examples of flavonoids and phenolcarbonic acids (Sigma-Aldrich). Accurately weighed quantities of standard identification samples were dissolved in appropriate volumes of methanol and rutin - to quantify in ethanol (70% (v / v)). For quantitative determination of flavonoids, they used the differential spectrophotometry method with a spectrophotometer Cary-50. Content of flavonoids was converted in rutin. Applying modern approaches to standardization of medicinal plants, they processed methods of identification and quantification of flavonoids in Pyrola rotundifolia leaf. An identification criterion to be incorporated into the quality control technique applicable to medicinal plants is TLC determined flavonoids. They substantiated and experimentally proved that a quantitative indicator of Pyrola rotundifolia leaf is flavonoid content, a quantitative criterion of which still to be determined through analysis of more samples. Based on modern approaches to standardization of herbal medicines, they have processed methods for identification and quantification of flavonoids in medicinal plants – Pyrola rotundifolia leaf. They offered incorporating the TLC-based flavonoids identification method into the quality control procedures applicable to Pyrola rotundifolia leaf; identification markers shall be availability of four flavonoids, including hyperoside and luteolin-7-O-glucoside, and three phenol-glycosides, which may be arbutin derivatives. Methods to control quality of medical plant shall include quantitative determination of flavonoids by the spectrofluorimetric method. However, to establish quantitative criterion, further research is required using more samples of Pyrola rotundifolia leaf from different vegetation regions.