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Vol. 6, Issue 7 (2017)

Effect of extender and different storage temperature on keeping quality of crossbred LWY boar semen

Author(s):
Mahesh M, Chandrashekar Reddy K, Ramchandra Reddy K, Sadanand D Sontakke and Solmon Raju KG
Abstract:
Comparative efficacy of Farmer friendly Boar Semen Extender (FBSE), Normal Boar Semen Extender (NBSE) and PRIMXcell extender for preservation of boar semen up to 96 h was studied. Extended semen was stored at 5 0C in FBSE and at 15-18 0C in NBSE and PRIMXcell extenders. A total of 32 ejaculates (8 from each boar) from 4 crossbred LWY boars were collected. The overall mean per cent of individual motility, Live spermatozoa, Acrosome integrity, Hypo osmotic Swelling Test reacted spermatozoa and Morphologically normal spermatozoa of the semen after storage of 96 h in FBSE, NBSE and PRIMXcell was 67.65 ± 1.11, 60.00 ± 1.33 and 26.95 ± 2.03; 74.98 ± 0.77, 69.38 ± 1.00 and 58.35 ± 1.48; 90.10 ± 0.38, 91.20 ± 0.32 and 78.65 ± 0.82; 32.95 ± 1.06, 30.95 ± 1.13 and 12.38 ± 1.11 and 83.96 ± 0.38, 85.99 ± 0.40 and 87.40 ± 0.36, respectively. Overall mean pH of the extended semen was 6.67 ± 0.04, 6.87 ± 0.04 and 7.98 ± 0.03 in FBSE, NBSE and PRIMXcell extenders respectively. Individual motility and live spermatozoa were significantly (p≤ 0.01) higher in FBSE followed by NBSE, significantly low in PRIMXcell extender. The acrosome integrity and HOST reacted spermatozoa in FBSE and NBSE extenders were significantly (p≤ 0.01) differed from PRIMXcell extender. Significantly (p≤ 0.01) higher per cent of morphologically normal spermatozoa were observed in PRIMXcell extender followed by NBSE and FBSE. FBSE was found to be the superior extender for preservation followed by NBSE.
Pages: 1011-1014  |  522 Views  13 Downloads
How to cite this article:
Mahesh M, Chandrashekar Reddy K, Ramchandra Reddy K, Sadanand D Sontakke, Solmon Raju KG. Effect of extender and different storage temperature on keeping quality of crossbred LWY boar semen. Pharma Innovation 2017;6(7):1011-1014.
The Pharma Innovation Journal