Vol. 6, Issue 7 (2017)

Author(s):
Pottabathula Mallesh, Manchukonda Udaya Kumar And Gss Murthy

Abstract:
In the present study morphology and different staining techniques such as Methyl green pyronin (MGP) staining, Giemsa staining and Formalin Azocarmine Alcohol Lactophenol (FAAL) staining were used to visualize the structure of salivary gland of Argas persicus. The paired elongate salivary glands of male and female Argas persicus comprised of 2 types of alveoli viz., type I and type II. The type I alveoli were oval or pear shaped consisting of peripheral and central zones. Type II alveoli had circular or oval outline and consisted of three types of cells surrounding a central lumen and lying on a fine basement membrane In MGP staining, the type I alveoli appeared as pink colour with a centrally located large oval nucleus. In Giemsa staining, the type I alveoli appeared in purple colour with centrally located large oval nucleus. In FAAL staining both type I and II alveoli appeared in orange colour. Type I alveoli showed orange coloured large oval nucleus. Triangular cells were seen in type II alveoli. As compared to MGP and Giemsa stain, the FAAL stain was better to visualize the structure of salivary glands.