Vol. 6, Issue 7 (2017)

Author(s):
Pottabathula Mallesh, Udaya Kumar, Gss Murthy And M Lakshman

Abstract:
In the present study the checker board titration was applied for optimization of Goat anti-chicken IgY HRPO conjugate, Argas persicus salivary gland antigen concentration and serum dilution. In the present study, the optimum conjugate dilution was 1:3000, optimum antigen concentration was 1 µg and optimum serum dilution was 1:100. The overall sensitivity of ELISA was 84.78% (39 out of 46 true positives) and the specificity as 70% (7 out of 10 true negatives). The laboratory standardized ELISA was applied on chicken sera collected from different poultry farms located in and around Hyderabad. A total of 486 chicken sera (326 layers and 160 backyard chicken) were tested out of which 125 (119 commercial layers plus 6 backyard chicken) were found positive indicating 25.72% (36.50% in commercial layers and 3.75% in backyard chicken) of prevalence respectively. A significant difference existed between the incidences among layers and backyard chicken.