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Vol. 6, Issue 7 (2017)

Standardization of enzyme linked immunosorbent assay for the detection of antibodies to Argas persicus (Oken, 1818) infestation in fowl of Telangana State

Author(s):
Pottabathula Mallesh, Udaya Kumar, Gss Murthy And M Lakshman
Abstract:
In the present study the checker board titration was applied for optimization of Goat anti-chicken IgY HRPO conjugate, Argas persicus salivary gland antigen concentration and serum dilution. In the present study, the optimum conjugate dilution was 1:3000, optimum antigen concentration was 1 µg and optimum serum dilution was 1:100. The overall sensitivity of ELISA was 84.78% (39 out of 46 true positives) and the specificity as 70% (7 out of 10 true negatives). The laboratory standardized ELISA was applied on chicken sera collected from different poultry farms located in and around Hyderabad. A total of 486 chicken sera (326 layers and 160 backyard chicken) were tested out of which 125 (119 commercial layers plus 6 backyard chicken) were found positive indicating 25.72% (36.50% in commercial layers and 3.75% in backyard chicken) of prevalence respectively. A significant difference existed between the incidences among layers and backyard chicken.
Pages: 174-179  |  690 Views  13 Downloads
How to cite this article:
Pottabathula Mallesh, Udaya Kumar, Gss Murthy And M Lakshman. Standardization of enzyme linked immunosorbent assay for the detection of antibodies to Argas persicus (Oken, 1818) infestation in fowl of Telangana State. Pharma Innovation 2017;6(7):174-179.
The Pharma Innovation Journal