Vol. 6, Issue 7 (2017)

Materials and Methods: Manjishtha root was evaluate to determine total, water soluble, acid insoluble ash & extractive values. Organic matter has determined the presence or absence of carbohydrate, alkaloids, amino acids, protein, saponin, glycosides, phenolic compound, tannins from aqueous & alcoholic extract. TLC of ethanolic extract has dropped on Silica Gel & Toluene: Ethyl Acetate: Formic Acid.

Results: 3 tests in Aqueous and 2 tests in alcoholic extract were found positive of carbohydrate. Alkaloids were found positive in 2 tests for aqueous extract & 2 tests from alcoholic extract. In both extract the amino acids was found positive which have major role in the detoxification of blood. Foam test was found positive in aqueous extract which is done for saponin which is a cleansing agent & washout the poison from vegetables. Keller kilini test was found positive only in aqueous extract which is done for glycosides. Phenolic compound was positive for both extract. Phenol plays a role as antiseptic along with anticarcinogenic, antioxidant properties. All the 4 tests in both extract were found positive which is done for tannins which play a major role as chemical antidote by precipitating most of the poisonous compound.

Conclusion: Carbohydrate, alkaloids, amino acids, saponin, glycosides, phenolic compound and tannins were found as a major constituent in Rubia cordifolia Linn. root which will play a major role as a antitoxin, detoxification of blood, antiseptic, antimutagenic, anticarcinogenic and antioxidant agent.