In terms of the progression of man-made pollution one of the priority areas of toxicology and medicine is the study of the characteristics and mechanisms of action of xenobiotics combined activity – risk factors of many ecologically dependent diseases. Among the most common xenobiotics – pesticides, nitrites and nitrates, heavy metals. Tobacco smoke belongs to the most aggressive "oxidative pollutants." It is known that in the basis of pathogenic effect of polluted by pollutants or tobacco smoke air is an oxidant aggression on the mucosa of the respiratory tracts with reactive oxygen species, leading to activation of lipid peroxidation and damage of biological membranes.
The aim of this work was to study the activity of free radical processes in rats of different ages poised with sodium nitrite against tobacco intoxication.
Experiments were performed on immature and mature rats that within 15 days were exposed to tobacco smoke affection. For 24 hours before the end of the experiment the animals of the group I were injected with sodium nitrite at a dose of 45 mg / kg of body weight, in group II – sodium nitrite was administered for 72 hours before euthanasia. Rats were taken out of the experiment under thiopental anesthesia. We use the general principles of experiments on animals approved and consistent with the provisions of the European Convention for the protection of vertebrate animals used for experimental and other scientific purposes when performing the research.
We found the probable increase in the content of reactive oxygen species in blood after poisoning by tobacco smoke leading to activation of lipid peroxidation and oxidative modification of proteins, accumulation of toxic intermediate products in the body and the development of oxidative stress. In all age groups of rats we observed a tendency to increase the content of lipid peroxidation and oxidative modification of proteins in the bodies of animals. Defeat of rats was complicated by application of additional toxicant – sodium nitrite. Immature and old rats were more sensitive to the simultaneous action of tobacco smoke and sodium nitrite, in which the content of TBA-active products and 2.4 dinitrophenylhydrazone significantly exceeded (r≤0.05) the level of intact control in blood serum, liver, lungs and heart.