Vol. 6, Issue 3 (2017)

Author(s):
Rajkishor Gogoi, Dulal Chandra Roy and Snehangsu Sinha

Abstract:
A study was undertaken to detect residues of Chlortetracycline in swine tissues using High Performance Liquid Chromatography (HPLC). About 300 nos. of representative swine tissue samples were collected from pork markets and roadside meat stalls located in and around Guwahati city of Assam. The samples after collection were preserved at -20 °C and then screened by High Performance Liquid Chromatography with UV-Vis Detector. Chlortetracycline residues were extracted with EDTA-McIIvaine buffer. Solid phase extraction clean up was done with SPE C18 cartridge. Recovery ranged from 94-98 %. About 8 nos. of the screened samples were detected to be positive for trace residues of chlortetracycline below the Maximum Residue Limit (MRL). It may be concluded that the screened samples were detected for residues well below the MRL.