Vol. 6, Issue 12 (2017)

Author(s):
K Yurkiv and A Grytsyk

Abstract:
We conducted the identification, separation and quantitative determination of phenolic compounds of European cranberry bushfruits using high performance liquid chromatography (HPLC). The compounds were separated on a Supelco Discovery C18 column. As a mobile phase we used solvent A which is 95% of the mobile phase mixture – 0.005 N orthophosphoric acid and 5 % of the solvent B – acetonitrile (for determination of phenolic compounds); solvent A which is 0.1% trifluoroacetic acid and 5% acetonitrile and water pH = 2.08 and 0.1% solvent B - trifluoroacetic acid and acetonitrile (for determination of tannins);solvent A - 0,005 N orthophosphoric acid and solvent B - acetonitrile (for determination of flavonoids). The highest content of epigallocatechin (0.56%), gallocatechin (0.20%) and epicatechin (0.17%), also chlorogenic acid (1.77%) was detected in Viburnum opulus L. fruits.