Cultural isolation and molecular characterization of etiological agents with reference to lamb mortality in Rayalaseema region of Andhra Pradesh, India
SV Raghavendra, A Anand Kumar, P Amaravathi, S Somasekhar Goud, Madhava Rao T and P Sudheer
The etiology for mortality in lambs may be due to infectious agents followed by non infectious origin. A total of 53 different samples were collected from ailing lambs (diarrhoea, nasal discharges, dullness, lambs with mixed signs) and during post mortem examination (showed suppurative pneumonia and abscesses on different organs) were inoculated into nutrient broth and incubated at 37 °C for 24 hrs. A loopful of inoculum from nutrient broth was streaked on different selective medias like EMB agar plates, MSA agar plates and BGA agar plates by following all the aseptic precautions. The plates were incubated at 37 °C for 48 hrs. The plates were observed for small dark colonies with green metallic sheen on EMB for E. Coli, pink to white colonies surrounded by red zone on BGA for Salmonella sp and yellow colonies on MSA for S. aureus. All the isolates were subjected to Gram’s staining and revealed that the isolates which were positive for Escherichia coli, Staphylococcus aureus and Salmonella sp were found gram negative coccobacillary rods, gram positive purple coloured cocci in clusters and gram negative bacilli respectively. Out of 32 E. coli isolates from different sources one (3.125%) isolate was found to be positive for the presence of stx2 gene at about 255 base pairs. Out of 7 cultures three (42.86%) isolates were found to be positive for Salmonella and positive isolates for the presence of invA gene at about 389 base pairs. Out of 29 cultures 23 (79.31%) isolates were found to be positive for S. aureus and positive isolates for the presence of nuc gene at about 279 base pairs.
How to cite this article:
SV Raghavendra, A Anand Kumar, P Amaravathi, S Somasekhar Goud, Madhava Rao T, P Sudheer. Cultural isolation and molecular characterization of etiological agents with reference to lamb mortality in Rayalaseema region of Andhra Pradesh, India. Pharma Innovation 2017;6(12):108-112.