Vol. 6, Issue 10 (2017)

Author(s):
Deepika Thandayuthapani, Nivetha Chinnappa, Arjunan Annavi, Sivalingam Periyasamy and Muthuselvam Manickam

Abstract:
This research is concerned about isolation, purification, characterisation and screening of bactericidal metabolite from the culture filtrate of Actinobacterium dagang 5. The 2.58 g of crude extract was obtained from 10 L of culture filtrate of Actinobacterium dagang 5 through ethyl acetate extraction. Later the concentrated crude extract was fractionated through silica gel column chromatography using hexane and ethyl acetate as eluting solvent system in gradient passion. The purified bactericidal compound having retention factor of 0.76 (8:2, hexane: ethyl acetate). The purified compound shows antibacterial activity against Klebsiella pneumonia, Vibrio cholerae, Proteus vulgaris. The compound was characterised and the structure was determined.