In vitro evaluation of indigenous formulation of Blepharis maderaspatensis (L.) B. Heyne ex Roth in the treatment of acute conjunctivitis
Jessy Jacob, NA Aleykutty and Jyoti Harindran
Ethanobotanical studies have revealed that eye drops prepared from Blepharis maderaspatensis (L.) B. Heyne ex Roth have been used for treating eye diseases. It was decided to test for the efficacy of both the hydro alcoholic extract as well as indigenous eye drop formulations in three different concentrations on the bacterial smears of acute conjunctivitis. The extracts of the concentration of 250,500,1000 ppm were prepared and evaluated for antibacterial activity. The indigenous formula using 200g of dry powdered plant drug powder for 400ml (0.5g/ml) of sesame oil did not show a significant anti microbial activity. So doses of 1g /ml and 1.5g/ml were prepared and evaluated. It was seen that the eye drops containing the second dose did show some activity but were not comparable to the standard .The eye drops of the next higher dose showed activity comparable to that of the standard drug .The extracts showed comparable activity at the concentration of 1000µg/ml. So, thereby authenticating the concept of use of eye drops of Blepharis maderaspatensis (L.) B. Heyne ex Roth for eye diseases like acute bacterial conjunctivitis.
How to cite this article:
Jessy Jacob, NA Aleykutty, Jyoti Harindran. In vitro evaluation of indigenous formulation of Blepharis maderaspatensis (L.) B. Heyne ex Roth in the treatment of acute conjunctivitis. Pharma Innovation 2017;6(10):240-242.