To evaluate the genotoxicity of gastric proton pump inhibitor omeprazole and role of vitamin-E in mice.
Materials and Methods: Forty two male albino mice were randomly divided into seven groups (n=6).Group I-control group which was served with distill water. In groups (II-IV) mice were treated with omeprazole i.e., 1000(1/4LD50), 2000(1/2LD50) and 3000(3/4LD50)mg/kg body wt orally for two days in two divided doses. Estimation of role of vitamin E in genotoxicity was done in groups (V, VI, VII) & Mice were treated initially with vitamin E with 100mg/kg body wt orally per day for six days, then omeprazole was given for two days in two divided doses(1/4, 1/2, and ¾ LD50) after 6hrs of the last dose of omeprazole animals in all groups were sacrificed by cervical dislocation and bone marrow cells were aspirated, smear was made and staining was done, screened under microscope for the presence of micronuclei in bone marrow cells.
Results: The results were obtained by applying student’s t-test. In the present study with micronucleus assay genotoxic effect of omeprazole with ¼, 1/2 ¾ LD50 doses was observed by the increase in the incidence of micronuclei in polychromatic and total erythrocytes in groups-II III and IV than group I and protective effect of vitamin E was observed by decrease in the incidence of micronuclei in polychromatic and total erythrocytes comparison to groups II, III and IV with the doses of omeprazole +vitamin E (100mg/kg body wt).
Conclusion: Omeprazole is having the genotoxicity effect on bone marrow cells with all the 3doses ¼, ½ and ¾ LD50 and vitamin E has got the protective effect for all the doses of omeprazole on the bone marrow.