Histological and SEM studies on somatic embryogenesis in rhizome- derived callus of Panax assamicus. Ban.
Ladaplin Kharwanlang, Meera C. Das, Suman Kumaria, Pramod Tandon
Somatic embryogenesis is an important technique of plant biotechnology in medicinally important plants. Ginseng which is the common name referred to species of the genus Panax (Araliaceae), has been valued as a potentiating oriental herbal medicine. Somatic embryogenesis of Panax assamicus Ban. was initiated from approximately four-five years old rhizome explant through callogenesis. Highest callus proliferation was observed in MS medium supplemented with 5 mg/L 2,4-D. Eight weeks old callus on being transferred to MS medium supplemented with 5mg/L 2,4-D and 0.5mg/ L BAP in combination showed best result in embryogenic calli which eventually formed somatic embryos. Induction of somatic embryos occurred after 3 months of sub culture in the same medium. Highest germination of somatic embryos occurred in 1/2MS medium incorporated with 1.5mg/L gibberellic acid. To confirm the appearance of the embryo-like structures in greater detail, the structures were observed microscopically by scanning electron microscopy and through histological sections. Complete normal plantlets were regenerated within 4 to 5 months after the induction of somatic embryos Plantlets were acclimatized successfully to ex vitro conditions with the survivability of 70% in the green house.
How to cite this article:
Ladaplin Kharwanlang, Meera C. Das, Suman Kumaria, Pramod Tandon. Histological and SEM studies on somatic embryogenesis in rhizome- derived callus of Panax assamicus. Ban.. Pharma Innovation 2016;5(4):93-99.