Blood glucose control remains a main focus in the managing of patients with type 2 diabetes. Pioglitazone is one of the insulin sensitizer with limitations like weight gain and peripheral edema, increase the incidence of heart failure and increase the risk of bone fractures. To this purpose we evaluated anti hyperglycemic activity of Ocimum sanctum Linn leaf in comparison with Pioglitazone in alloxan induced diabetic rats.
Methods: This comparative study was conducted in 16 male Wister rats for the period of 3 months. Ocimum leaves were dried; Soxhlet apparatus was used for extraction. Diabetes mellitus was induced in rats by single intraperitoneal injection of Alloxan monohydrate dissolved in sterile normal saline at a dose 160 mg/kg body weight. DM mellitus was confirmed by measuring fasting blood glucose level using tail vein blood sample. After 1 week, rats with fasting blood glucose of more than 300 mg/dl were considered as diabetics and used for further experiment. The data was expressed as mean ± SEM, Statistical difference in mean were analyzed by one way ANOVA.* P <0.05 was considered as significant.
Results: Administration of the test drug i.e aqueous extract of Ocimum sanctum Linn (300 microgram/gram body weight), produced statistically significant reduction in the blood glucose level (p <0.0001) which was 345 mg/dL before treatment and reduced to 263 mg/dL after treatment.
Conclusion: Aqueous extract of leaves of Ocimum sanctum Linn has got significant anti-diabetic activity by its antihyperglycemic action and insulin sensitizing activity greater than control and lesser than reference drug pioglitazone.