Vol. 4, Issue 4 (2015)

Author(s):
Mohammad Nazmul Alam, Md. Shahrear Biozid, Md. Rafikul Islam, Md. Masudur Rahman, Ahmad Ibtehaz Chowdhury, Muhammad Moin Uddin Mazumdar

Abstract:
Objective: To evaluate comparative study of anti-inflammatory, anti-arthritic activity of methanol extract of Cissus pentagona Roxb. (Vitaceae.) and Thunbergia grandifloraRoxb. (Acanthaceae) leaf.
Methods: Human Red Blood Cell (HRBC) membrane stabilization method was evaluated for anti-inflammatory activity. Anti-denaturation method was performed by using bovine serum albumin (BSA) to evaluate the anti-arthritic potential.
Results: The in vitro anti-inflammatory activity of the methanol extracts of C. pentagona andT. grandiflora showed 78.89% and 89.11% of membrane stabilization at 1000 µg/ml conc and 42.22% and 63.37% at 31.25 µg/ml respectively. All the results were compared with standard Diclofenac which showed 94.44% protection at 1000 µg/ml conc. The in vitro study on both leaves also showed the presence of significant anti-arthritic activity. Here the extracts of C. pentagona and T. grandiflora showed 69.35% and 74.19% of protein denaturation at highest conc (1000 ug/ml) and 38.71% and 45.16% at lowest conc (31.25 ug/ml) respectively, in where the standard drug showed the 85.49% at 1000 ug/ml and 51.61% at 31.25 ug/ml conc.Conclusion: These result suggested that both the methanol extract of C. pentagona Roxb. and T. grandiflora Roxb. contains anti- inflammatory and anti-arthritic activity.