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Vol. 3, Issue 6 (2014)

Characterization of Fructosyltransferase Produced by Syncephalastrum racemosum Cohn

M.B. Patil and Ashwin Butle
An indigenously isolated strain Syncephalastrum racemosum Cohn was found to produce fructosyltransferase (Ftase) enzyme. The Ftase was purified by conventional purification techniques to homogeneity. SDS PAGE revealed the molecular weight of the purified Ftase to be 37 KD. Optimum pH for activity was 5.5 while an optimum temperature was recorded to be 55oC. Mg++ and Mn++ were able to restore the activity of the metal chelator treated purified Ftase. The Ftase was found to be glycoprotein in nature. PMSF, PCMB, DTNB, β-mercaptoethanol and iodoacetate did not inhibit the activity completely, but the surfactants were able to inhibit the activity completely.
Pages: 87-91  |  1290 Views  7 Downloads

The Pharma Innovation Journal
How to cite this article:
M.B. Patil, Ashwin Butle. Characterization of Fructosyltransferase Produced by Syncephalastrum racemosum Cohn. Pharma Innovation 2014;3(6):87-91.
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