Vol. 2, Issue 12 (2014)

Author(s):
T. Sruthi*, D.Satyavati, Ch. Maheswara Reddy, N.Srilatha

Abstract:
The objective of present study was focused to estimate the glycogen content in liver, skeletal muscle and cardiac muscle of NIDDWIN in diabetic rats. Alloxan induced diabetic rats were divided into four groups of five each. Group-I was given 2% gum acacia, Group-II and III was given NIDDWIN 50mg/kg and 100mg/kg, Group-IV was given Glibenclamide 10mg/kg were given orally to each group, 2hours after administration of drugs into different groups they were scarified liver, skeletal muscle, cardiac muscle were isolated and taken into test tube containing 5% trichloroaceticacid with 5ml ethanol and allowed to stand for overnight at room temperature and the precipitate obtained is estimated for glycogen content. NIDDWIN 100mg/kg showed significant to Glibenclamide 10mg/kg in increasing the glycogen content in muscles. NIDDWIN concluded that it possess reduction in blood glucose levels by increasing glycogen synthesis as that of Glibenclamide.