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Vol. 9, Issue 1 (2020)

Molecular Identification of Str A gene responsible for streptomycin production from Streptomyces isolates

Author(s):
Bayader Abdel Mohsen, Mohsen Hashim Risan and Asma G Oraibi
Abstract:
The present study was aimed for molecular identification of Str A gene from Streptomyces isolates. Twenty-four isolates were identified as Streptomyces sp. based on their morphological and biochemical characteristics. Twelve isolates were positive in the PCR technique. Performing PCR reactions using primer pair on DNA. The results of Str A gene detection clarify that Two isolate of Streptomyces isolates gave a positive result and carrying Str gene, while 10 of Streptomyces isolates were lacking the gene. Be1 and B3-4 isolates gave DNA bands 700 bp in length. The results indicated that the Be1 and B3-4 isolates are very close to the species Streptomyces griseus responsible for producing antibiotic streptomycin.
Pages: 18-24  |  175 Views  8 Downloads
How to cite this article:
Bayader Abdel Mohsen, Mohsen Hashim Risan and Asma G Oraibi. . The Pharma Innovation Journal. 2020; 9(1): 18-24.
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